Enhancement and statistical optimization (Plackett-Burman and response surface methodology) of biosurfactant production by marine bacteria Bacillus tequilensis KM15 using Fish processing wastes

Biosurfactant (BS) production by Bacillus tequilensis KM15 is investigated using fish processing wastes as medium components. Bocha (B) and Rohu (RR) scales are selected cost-effective substrates for BS optimized together with pH, the incubation period (IP), inoculum size (IS) response surface method (RSM). RSM model has demonstrated following optimum conditions production: 4% each, 2%, at pH 8.0, 37 ̊C temperature in 48 h. A 2.8-fold increase biosurfactant was obtained (from 340 mg L-1 basal to 944 L-1) methodology. The major portion of cost can be reduced currently studied substrates.